restriction site造句
例句與造句
- In this case , two restriction sites ( ecor i , hind iii ) were added to the primers ( p1 , p2 ) separately
擴(kuò)增產(chǎn)物經(jīng)雙酶切后，重組到表達(dá)載體pet - 32a ( + )中。 - Oligomap - oligomap is a tool to help researchers engineer restriction sites into oligonucleotides using silent mutagenesis and reverse translation algorithms
Oligomap是一個(gè)幫助研究人員使用啞誘變和反向轉(zhuǎn)換算法來約束寡核苷酸位置點(diǎn)的工具。 - 2 . ecori / bamhi digested pcr products were inserted into the corresponding restriction site in the expression plasmid pbv220 . construction of non - fusion expression plasmid pbv220 - endostatin
用ecori和bamhi酶切endostatincdna的pcr產(chǎn)物，將其插入到質(zhì)粒載體pbv220中相應(yīng)的限制性酶切位點(diǎn)，構(gòu)建非融合質(zhì)粒表達(dá)載體pbv220 - endostatin 。 - A pair of primers containing sph i and hind iii restriction sites were designed , according to the poifn - a gene in ddbj / genbank . then poifn - a gene was cloned from porcine genomic dna by pcr
根據(jù)ddbj genbank基因庫中已登錄的豬干擾素基因序列，設(shè)計(jì)了含sph和hind酶切位點(diǎn)的一對引物，采取聚合酶鏈?zhǔn)椒磻?yīng)( pcr )法，以豬基因組dna為模板進(jìn)行了poifn克隆。 - Ba - dfe mature peptide - coding sequence was cloned to the restriction site ncol and ndei of the expression vector pet - 15b , respectively , and highly expressed in e . coli bl21 ( de3 ) by the form of non - fusion and his - badfe fusion protein
Lmmol liptg誘導(dǎo)時(shí)，融合表達(dá)時(shí)產(chǎn)生分子量約為30kd 、帶有his標(biāo)簽的ba dfe融合蛋白，非融合表達(dá)時(shí)則產(chǎn)生分子量為28kd的ba dfe 。 - It's difficult to find restriction site in a sentence. 用restriction site造句挺難的
- 6 . the restriction analysis of its amplified product showed that no restriction site was observed for bamh i in all isolates , the other tested enzymes ( alu , hae iii , hinf i , taq i , hha i , msp i ) could distinguish p . cilrinopileatus from the other pleurotus species
6 . [ ts擴(kuò)增產(chǎn)物的限制性酶切分析結(jié)果表明，召til ) ， hl對所有供試菌株均無酶切位點(diǎn)，而另外6利，供試內(nèi)切酶( alul 、 haeitl 、 llllal 、 11infl 、九式斗， i 、 tcl叮i )都能將金頂側(cè)耳與其它側(cè)耳菌株區(qū)分開。 - Total rna was extracted from hepatic cells of mouse . a ecori and bamhi restriction sites were introduced into endostatin gene at specific primer f r , and endostatin was amplified by rt - pcr , this endostatin gene contained bamhi and ecori restriction sites at its 5 " and 3 " ends respectively
從小鼠肝臟細(xì)胞中提取總rna 。設(shè)計(jì)合成一對特異引物，分別帶有ecori和bamhi的限制性內(nèi)切酶的識別位點(diǎn)。用rt - pcr法擴(kuò)增endostatin的基因片段，在endostatin基因的兩側(cè)引入ecori和bamhi酶切位點(diǎn)。 - Sinensis and e . j . hepuensis has been found in the sequences of the portions of 16s rdna and pcr / rflp studies of 110 samples , from six river valleys in eastern mainland of china . these subspecies - specific restriction sites allow rapid discrimination with the endonuclease dra i , and therefore can be used as a diagnostic genetic marker for identification of the two subspecies
通過對中國大陸東部6個(gè)水系110個(gè)絨螯蟹個(gè)體16srdna部分序列的測定和pcr rflp分析，發(fā)現(xiàn)在合浦絨螯蟹與中華絨螯蟹之間存在3 4個(gè)固定的堿基替代，這種亞摘要種特異性的限制性位點(diǎn)可以通過限制性內(nèi)切酶dra進(jìn)行快速檢測，成為2個(gè)亞種的分子鑒定標(biāo)記。 - 2 . construction of chimeric mtb8 . 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8 . 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8 . 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments , respectively
3 .重組質(zhì)粒在真核細(xì)胞中的表達(dá): pm 、 pms 、 pmi和pmsl重組質(zhì)粒用lipofectaminatmzo0o脂質(zhì)體轉(zhuǎn)染試劑轉(zhuǎn)染cos一7細(xì)胞，進(jìn)行瞬時(shí)表達(dá)， 48小時(shí)后，用rl ’ - pcr檢測目的基因在mrna水平的表達(dá);用westemblotting檢測hil一12在蛋白質(zhì)水平的表達(dá)。